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plasmid pdge463 carrying expression cassettes  (Addgene inc)


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    Addgene inc plasmid pdge463 carrying expression cassettes
    Plasmid Pdge463 Carrying Expression Cassettes, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plasmid+pdge463+carrying+expression+cassettes/pDGE463+(Plasmid+%23153234)/bio_rxiv__2025__10__01__679797-116-16-38
    Average 93 stars, based on 1 article reviews
    plasmid pdge463 carrying expression cassettes - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Gene editing of Nicotiana benthamiana architecture for space-efficient production of recombinant proteins in controlled environments
    Article Snippet: .. Plasmid pDGE331 harboring the Arabidopsis U6 promoter was used as shuttle vector for either sgRNAs, and plasmid pDGE463 carrying expression cassettes for Cas9, the CcdB toxin and the NptII selection marker as a recipient vector for plant transformation (AddGene) ( ). sgRNA cassettes were inserted by Golden Gate cloning into the recipient vector by ccdB excision with BsaI. .. The assembled vectors were cloned into Escherichia coli strain DH5α, extracted with the QIAprep Spin Miniprep Kit (QIAGEN), and verified by Sanger sequencing with the M13F-47 primer to confirm insertion of the sgRNA cassette.

    Expressing:

    Article Title: Gene editing of Nicotiana benthamiana architecture for space-efficient production of recombinant proteins in controlled environments
    Article Snippet: .. Plasmid pDGE331 harboring the Arabidopsis U6 promoter was used as shuttle vector for either sgRNAs, and plasmid pDGE463 carrying expression cassettes for Cas9, the CcdB toxin and the NptII selection marker as a recipient vector for plant transformation (AddGene) ( ). sgRNA cassettes were inserted by Golden Gate cloning into the recipient vector by ccdB excision with BsaI. .. The assembled vectors were cloned into Escherichia coli strain DH5α, extracted with the QIAprep Spin Miniprep Kit (QIAGEN), and verified by Sanger sequencing with the M13F-47 primer to confirm insertion of the sgRNA cassette.

    Selection:

    Article Title: Gene editing of Nicotiana benthamiana architecture for space-efficient production of recombinant proteins in controlled environments
    Article Snippet: .. Plasmid pDGE331 harboring the Arabidopsis U6 promoter was used as shuttle vector for either sgRNAs, and plasmid pDGE463 carrying expression cassettes for Cas9, the CcdB toxin and the NptII selection marker as a recipient vector for plant transformation (AddGene) ( ). sgRNA cassettes were inserted by Golden Gate cloning into the recipient vector by ccdB excision with BsaI. .. The assembled vectors were cloned into Escherichia coli strain DH5α, extracted with the QIAprep Spin Miniprep Kit (QIAGEN), and verified by Sanger sequencing with the M13F-47 primer to confirm insertion of the sgRNA cassette.

    Marker:

    Article Title: Gene editing of Nicotiana benthamiana architecture for space-efficient production of recombinant proteins in controlled environments
    Article Snippet: .. Plasmid pDGE331 harboring the Arabidopsis U6 promoter was used as shuttle vector for either sgRNAs, and plasmid pDGE463 carrying expression cassettes for Cas9, the CcdB toxin and the NptII selection marker as a recipient vector for plant transformation (AddGene) ( ). sgRNA cassettes were inserted by Golden Gate cloning into the recipient vector by ccdB excision with BsaI. .. The assembled vectors were cloned into Escherichia coli strain DH5α, extracted with the QIAprep Spin Miniprep Kit (QIAGEN), and verified by Sanger sequencing with the M13F-47 primer to confirm insertion of the sgRNA cassette.

    Transformation Assay:

    Article Title: Gene editing of Nicotiana benthamiana architecture for space-efficient production of recombinant proteins in controlled environments
    Article Snippet: .. Plasmid pDGE331 harboring the Arabidopsis U6 promoter was used as shuttle vector for either sgRNAs, and plasmid pDGE463 carrying expression cassettes for Cas9, the CcdB toxin and the NptII selection marker as a recipient vector for plant transformation (AddGene) ( ). sgRNA cassettes were inserted by Golden Gate cloning into the recipient vector by ccdB excision with BsaI. .. The assembled vectors were cloned into Escherichia coli strain DH5α, extracted with the QIAprep Spin Miniprep Kit (QIAGEN), and verified by Sanger sequencing with the M13F-47 primer to confirm insertion of the sgRNA cassette.

    Cloning:

    Article Title: Gene editing of Nicotiana benthamiana architecture for space-efficient production of recombinant proteins in controlled environments
    Article Snippet: .. Plasmid pDGE331 harboring the Arabidopsis U6 promoter was used as shuttle vector for either sgRNAs, and plasmid pDGE463 carrying expression cassettes for Cas9, the CcdB toxin and the NptII selection marker as a recipient vector for plant transformation (AddGene) ( ). sgRNA cassettes were inserted by Golden Gate cloning into the recipient vector by ccdB excision with BsaI. .. The assembled vectors were cloned into Escherichia coli strain DH5α, extracted with the QIAprep Spin Miniprep Kit (QIAGEN), and verified by Sanger sequencing with the M13F-47 primer to confirm insertion of the sgRNA cassette.



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    plasmid pdge463 carrying expression cassettes  (Addgene inc)
    93
    Addgene inc plasmid pdge463 carrying expression cassettes
    Plasmid Pdge463 Carrying Expression Cassettes, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plasmid+pdge463+carrying+expression+cassettes/pDGE463+(Plasmid+%23153234)/bio_rxiv__2025__10__01__679797-116-16-38
    Average 93 stars, based on 1 article reviews
    plasmid pdge463 carrying expression cassettes - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier

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